mouse monoclonal antibody against human α syn  (Novus Biologicals)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Protective effects of paederoside in rotenone-induced cellular models of Parkinson’s disease

doi: 10.3389/fcell.2025.1631652

Figure Lengend Snippet: Effect of paederoside on α-Syn nitration triggered by rotenone in an N2A/BV-2 co-culture model (n = 3). (A) Conrol group; (B) Rotenone group; (C) Rotenone+0.1 micromolar (μM Paederoside; (D) Rotenone+1 μM Paederoside; (E) Rotenone+10 μM Paederoside; (F) 20 nM rotenone treatment resulted in a significant increase in nitrated α-Syn in the N2A/BV-2 cell co-culture model; (G) Paederoside significantly reduced the levels of nitrated α-Syn induced by rotenone in an N2A/BV-2 co-culture model. *P < 0.05 as compared with rotenone-treated group and # P < 0.05 as compared with control group.

Article Snippet: The primary mouse monoclonal antibody against human α-Syn (nitrated Tyr125, nitrated Tyr133) was procured from Novus Biologicals.

Techniques: Nitration, Co-Culture Assay, Control

Journal: NPJ Parkinson's Disease

Article Title: Nigral ATP13A2 depletion induces Parkinson’s disease-related neurodegeneration in a pilot study in non-human primates

doi: 10.1038/s41531-024-00757-4

Figure Lengend Snippet: a Representative nigral sections of endogenous α-syn immunostaining (top) and scatter plot (bottom) of the mean grey value of syn211 immunostaining in the substantia nigra of control and shATP13A2-injected NHPs by quantification of surface staining, expressed as a percentage of controls macaques ( p = 0.16355, t = 1.067). Scale bar: 200 µm. b Illustrative photomicrographs (top) and quantification (bottom) of phosphorylated S129 α-synuclein in the SNpc of control and shATP13A2-injected NHPs ( p = 0.0109, t = 3.077). Scale bars = 100 µm (sections) and 10 µm (insets). c Midbrain protein extracts were analysed by dot-blot assay on nitrocellulose membrane and revealed by immunoblot with syn ( p = 0.0528, t = 1.972) and pSyn ( p = 0.0001, t = 13.39) antibodies. d Midbrain protein extracts were treated with proteinase K (PK) (0.1 µg/ml) for 0, 15, 30 and 60 min and analyzed by dot-blotting with syn211 antibody. Each dot represents one hemisphere of the control (black) and shATP13A2-injected NHPs (red) (light red is one macaque/deep red is the second macaque). Bootstrapped mean difference with 95% CI (error bar) is shown on the right side of each graph. Comparisons were made using an unpaired t -test. * p < 0.05 compared to control animals.

Article Snippet: α-synuclein status was assessed with a mouse monoclonal antibody raised against human α-syn (ThermoFisher [Syn211], 32-8100, 1:1000) and another against phosphorylated α-syn (Abcam, [EP1536Y], ab51253, 1:5000) as previously described , , .

Techniques: Immunostaining, Control, Injection, Staining, Dot Blot, Membrane, Western Blot

Journal: NPJ Parkinson's Disease

Article Title: Nigral ATP13A2 depletion induces Parkinson’s disease-related neurodegeneration in a pilot study in non-human primates

doi: 10.1038/s41531-024-00757-4

Figure Lengend Snippet: Heat map representing the normalized expression (Z scoring from control values) on the different variables used in this study, for each hemisphere of the two experimental NHPs. Controls are pulled as mean. From left to right: stereological counting Tyrosine Hydroxylase (TH)-positive neurons in the substantia nigra (SN) (stereo TH SN), TH, AADC and DAT immunostaining levels measured in the putamen and in the caudate nucleus (TH putamen; TH caudate; AADC putamen; AADC caudate; DAT putamen; DAT caudate), α-syn and pSyn signals in the SN (syn211 SN, pSyn SN, DB syn211 SN, DB pSyn SN, ratio pSyn/Syn211), the average number of LAMP2-positive puncta and LC3-positive puncta in the whole cell, perinuclear and cytosolic areas (LAMP2 nb whole cell, LAMP2 nb perinuclear, LAMP2 nb cytosolic, LC3 nb whole cell, LC3 nb perinuclear, LC3 nb cytosolic), the heavy metals content in SN (Iron, Zinc, Copper, Calcium, Manganese, Sulfur). The colour bars represent the z-score value of the ratio of each brain region.

Article Snippet: α-synuclein status was assessed with a mouse monoclonal antibody raised against human α-syn (ThermoFisher [Syn211], 32-8100, 1:1000) and another against phosphorylated α-syn (Abcam, [EP1536Y], ab51253, 1:5000) as previously described , , .

Techniques: Expressing, Control, Immunostaining